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Subsequently, a 200-?l fixation buffer was used to resolve the cells for 20 min. Right after washing the cells 3 times with precooled PBS, 1× intracellular staining permeabilization clean buffer that contains 1% DAPI (blue) was placed on mark the nuclei of your cells. 10 minutes later on, the cells had been washed three times with precooled PBS a
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